Isolation and Identification of Bacteria Causing Gastroenteritis

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Block B3 - Abdominal Problems

Office:4th floor, Radiopoetro.
Lab:4th floor, Radiopoetro.
Pre-test:10 questions, true-false.
Lab Report:None.
Lab session observations
Lab session: test-tubes
E.Coli growing in Motility Indole Ornithine (MIO) medium with a few drops of Kovacs reagent


Batch 2011[edit]

  1. EHEC is a common cause of "traveler's diarrhea". False.
  2. Pseudomonas is a member of Enterobacteriaceae. False.
  3. Enterobacteriaceae are gram-positive. False.
  4. Enterobacteriaceae can be differentiated by microscopy. False.
  5. TCBS is used for the isolation of Vibrio sp. True.
  6. V. cholerae shows yellow colonies. True.
  7. MIO is used to see motility. True.
  8. Alkaline pepton water is used as a transport medium for vibrio. True.
  9. Proteus sp. form red colonies. False.
  10. Lactose-fermenting bacteria form white colonies. False.

Batch 2017[edit]

  1. Pseudomonas is NOT an enterobactericeae. True
  2. E. Coli is an endogenous bacteria. True
  3. Indole is made out of lysine. False
  4. Lactose fermenting bacteria gives pink-pigmented colonies. True
  5. Salmonella sp. is a non-lactose fermenting bacteria. True
  6. ETEC is the cause of non-bloody diarrhea. True
  7. EHEC is the cause of bloody diarrhea. True
  8. SSS and MIO is used to detect motility. True
  9. Vibrio Cholerae in TCBS prpduce red color. False
  10. Enterobactericeae is a short gram negative cocci. False

Credits to: Quency