Methods for Isolation, Identification of Anaerobic Bacteria, and Anaerobic Bacteria Causing Myonecrosis

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Block B2 - Neuromusculoskeletal Problems

Office:4th floor, Radiopoetro.
Lab:4th floor, Radiopoetro.
Pre-test:10 questions, true-false.
Lab Report:None.
20140311 104701.jpg
Spore stain procedure
Spore attached to bacteria (rods) under 1000x magnification
Anaerobic and aerobic bacteria: aerobic prefers air, so it is concentrated near the top.
Schaeffer–Fulton stain: endospores are stained green using malachite green, and the counterstain is safranin, which dyes any other bacterial bodies red.


Batch 2011[edit]

  1. Aerobic bacteria counters the negative (myonecrosis) effects of anaerobic bacteria.
  2. Gram positive rods can produce spores. True.
  3. Gram stain can be used to stain spores. True.
  4. Swab method can be used for specimen collection. False.
  5. C. perfringens is proteolytic. True.
  6. Myonecrosis can develop when open lesions come in contact with dirt.

Batch 2011 Regular[edit]

  1. Risk factors for infectious myonecrosis is mixed infection. True.
  2. C. Septicum can cause myonecrosis. True.
  3. GAM semisolid is anaerobic bacteria. True.
  4. The main virulence myonecrosis is alpha toxin. True.
  5. Midstream urine specimen can be used. False.
  6. Metronidazole is used in clostridium because it can generate color. False.
  7. C. perfringens is a proteolytic. True.
  8. C. perfringens is the main cause of myonecrosis. True.
  9. On examination grams clostridium is always shaped like a gram-negative cocci. False.
  10. Biochemical identification of clostridium can be used to see the fermentation of carbohydrates. True.

Batch 2010[edit]

  1. The stain used in Scaffer-Fulton method is Malachite green. True.
  2. Gram negative rod anaerobic bacteria can produce spores. False.


Batch 2011[edit]

  1. Name two anaerobic bacteria.
  2. C. perfringens can produce indole. false.
  3. C. butyricum can ferment all carbohydrates. true.
  4. What is this medium? brucella agar (with blood).
  5. Which one of these is non-proteolytic? C. cadaveris, C. bifermentans, C. difficile, C. tertium.