Microstructure of the Nervous System

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Block A5 - Nervous System and Sense Organs


Histology
Office:2nd floor, Anatomy
Lab:2nd floor, Anatomy
Work-plan:None.
Pre-test:5-6 questions, short-answer.
Post-test:5 questions, short answer.
Lab Report:None.
Cerebellum
Nissl bodies
Neurons and neuroglia
Cornu ammonis

Lab Session[edit]

In this session, basically learn to differentiate between the cerebellum and the cerebrum, white matter (few cells) and gray matter (lots of cells) areas, pia mater (stuck to the brain) and arachnoid mater, and neuronal cells and neuroglia (tiny cells).

For the post-test, know:

  • The layers for each of the specimens:
    • Cerebellum: molecular (contains stellate and basket cells, and dendrites from the purkinje cells), purkinje, granular. All three are part of the gray matter. Cerebellum is folded into folia. Proximal than the folia is the white matter where deep cerebellar nuclei can be found.
    • Cerebral cortex: molecular, ganglion (very thin layer of cells), granular. Cerebrum is folded into gyri and sulci.
    • Olfactory bulb: glomerulus (looks like empty circles/bubbles), mitral (thin layer of cells), granular (lots of cells).
  • Ependymal cells line the choroid plexus and are simple cuboidal in shape. They make CSF.
  • CTB stains pyramidal cells and their axons.
  • Meninges:
    • Dura mater: dense collagenous connective tissue. The space between dura and arachnoid is called subdural space.
    • Arachnoid mater: simple squamous, well vascularized. The space between arachnoid and pia is called subarachnoid space.
    • Pia mater: flattened, modified fibroblasts.
  • Toluidine blue stain is used to show Nissl bodies: found in rough endoplasmic reticulum (protein synthesis center) of the cell.
  • Cornu ammonis and gyrus dentatus. Also, the thalamus next to these structures.

Pretests[edit]

Answers[edit]

  1. Function of organelles (rough ER) that form Nissl bodies: production of proteins.
  2. Fresh basal ganglia is colored gray.
  3. What structure is found in the nuclei of cranial nerves? (Don't know. Maybe synapses? Nissl bodies?)